A method for determining the adenosine triphosphatase content of energy-transducing membranes. reaction of 4-chloro-7-nitrobenzofurazan with the adenosine triphosphatase of bovine heart submitochondrial particles

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

A method for determining the adenosine triphosphatase content of energy-transducing membranes. reaction of 4-chloro-7-nitrobenzofurazan with the adenosine triphosphatase of bovine heart submitochondrial particles.

1. Modification of a single amino acid residue by introduction of the nitrobenzofurazan group inactivates mitochondrial ATPase (adenosine triphosphatase) when membrane-bound in submitochondrial particles. The similarity between the reactions of both membrane-bound and isolated ATPase with 4-chloro-7-nitrobenzofurazan indicates that the single essential tryosine residue identified in the isolate...

متن کامل

Stimulation of Adenosine Triphosphatase Activity Mitochondria and Submitochondrial Particles

Crane and Lipmann (1) have shown that arsenate uncouples oxidative phosphorylation in intact rat liver mitochondria, and that this effect can be reversed by phosphate. They postulated that arsenate competes with phosphate at the site of phosphate uptake. It has also been observed that arsenate can stimulate the respiration of intact mitochondria (1) and of submitochondrial particles’ in the abs...

متن کامل

Spermine binding to submitochondrial particles and activation of adenosine triphosphatase.

Studies on the effects of polyamines on oligomycin-sensitive ATPase activity of ox heart submitochondrial particles showed that, of the polyamines tested, only spermine affected the enzyme activity. Spermine within the physiological concentration range increased the Vmax. of the enzyme, but the Km for ATP was virtually unaffected. Binding studies of [14C]spermine to submitochondrial particles, ...

متن کامل

Adenosine Triphosphatase Activity in the Membranes

This investigation deals with the localization of sites of ATPase activity, especially of transport ATPase, in nerve fibers of the squid Doryteuthis plei, at the subcellular level. Splitting of ATP liberates inorganic phosphate which reacts with lead to form a precipitate in the tissue. The reaction was made on nerve fibers fixed with glutaraldehyde. Frozen slices were incubated in Wachstein-Me...

متن کامل

Adenosine triphosphatase in isolated bacterial cell membranes.

Among the recognized morphological features of cells, the esternal membrane is one of the most poorly understood in terms of both structure and function, especially with regard to its participation in transport processes. In recent years, the chemical and enzymatic composition of isolated cell membranes from a few species of bacterial organisms have received the attention of a number of investi...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1976

ISSN: 0264-6021

DOI: 10.1042/bj1590347